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. 2018 Dec 28;47(5):2322–2335. doi: 10.1093/nar/gky1306

Figure 2.

Figure 2.

Ajuba enhances ERα transcriptional activity. (A) The pGL3.0-ERE-Luc reporter contains three tandem ERE elements and were transiently transfected into 293T cells. The resulting cells were treated with 20nM E2 or ethanol for 12hrs and were prepared for the luciferase reporter assay. The luciferase activity was normalized to the value of β-gal activity (three repeats, * means P < 0.05). (B and C) Wild-typed (B) or mutated (C) TFF1 promoter driven luciferase reporter plasmids were co-transfected with ERα and/or Ajuba plasmids into 293T cells. The transfected cells were treated with 20 nM E2 before being harvested for luciferase reporter assay and the value was normalized to β−gal (Three repeats, * means P < 0.05, NS means no significance). (D–F) The location of ERE in the promoter or enhancer of ERα target genes was shown in the upper panel. The T47D cells were treated with 100 nM E2 or ethanol for 1 h and prepared for the ChIP assay. The relative enrichment of Ajuba and ERα on the promoter or enhancer of TFF1 (D), Greb1 (E) and SGK3 (F) was detected by qPCR and normalized to input (three repeats, * means P < 0.05).