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. 2018 Dec 28;47(5):2322–2335. doi: 10.1093/nar/gky1306

Figure 4.

Figure 4.

Ajuba antagonizes the Tamoxifen-toxic effect in breast cancer cells. (A–C) Ajuba-overexpressed or knocked-down T47D and MCF7 cells were treated with TAM or ethanol for 6 days and cell viability was examined by CCK8 assay. The results were normalized to the normal control cells (ethanol-treated groups) (three repeats, * means P < 0.05, NS means no significance). (D–F) T47D-Ajuba and vector control cells were treated with TAM (100 nM) or ethanol for 4 days, the relative mRNA expression of ERα target genes was detected by qRT-PCR. The results were normalized by ethanol-control group and the inhibitory percent of TAM was labeled (three repeats, * means P < 0.05). (G–I) The effect of TAM (100 nM) on the expression of ERα target genes in MCF7-Ajuba and vector control cells were detected by qRT-PCR. The results were normalized by ethanol-control group and the inhibitory percent of TAM was labled (three repeats, * means P < 0.05). (J–L) Knocking-down Ajuba in T47D cells magnified inhibitory effect of TAM (100 nM) on the expression of ERα target genes. The results were normalized by ethanol-control group and the inhibitory percent of TAM was labeled (three repeats, * means P < 0.05).