Fig. 3.

Excessive secretion of PTH triggers adipose tissue browning in PHPT mice.

(a) Representative images of HE staining of adipose tissue sections in mice; scale bar 20 μm. (b) Representative images of the UCP1 staining of IWAT; scale bar, 50 μm for the left panels, and 20 μm for the right panels. (c-e) The relative mRNA level of thermogenic genes in IWAT (c), EWAT (d), BAT (e) of mice. 36b4 is used as the internal reference for normalization. Data are shown as Means ± SEMs; N = 10 and 12 in GFP and PHPT group, respectively. (f) The protein level of thermogenic genes in IWAT measured by western blot. Each lane represents protein sample from one mouse. Lane 1–5 are GFP group and lane 6–11 are PHPT group. Hsp90 is used as the internal reference for normalization. (g) The densitometric analysis of panel f. *P < .05, **P < .01, ***P < .001 vs GFP group.