Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 11;9:4114. doi: 10.1038/s41598-019-40904-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Activation of Rho GTPase by LET with siRNA. (a) Relative Rho GTPase activity. After 36 h of serum starvation, cells were treated with LET in presence of siRNA, and were lysed immediately after LET. Rho GTPase activity in the lysate was measured by G-Lisa activation assay (Cytoskeleton Inc.). (b,c) Cells pretreated with Rho inhibitor I for 4 h, followed by LET in presence of siRNA. (b) Effect of Rho inhibitor I on actin cytoskeleton remodeling induced by LET with siRNA. Red and blue signals indicate rhodamine phalloidin-labelled actin and nuclei, respectively. Scale bars indicate 20 μm. (c) The effect of Rho inhibitor I on LET-mediated cellular uptake of siRNA. Values represent the means of 3 individual experiments. Bars represent standard deviations. *P < 0.05 and ***P < 0.001.