Supplemental Fig. 2.

Liver Kinase B1 (LKB1) SUMOylation of mouse liver progenitor MLP-29 cells offers survival advantage during hypoxic stress. a. LKB1 overexpression was induced in human MLP-29 cell line by using the pcDNA3-FLAG-LKB1 Wild Type plasmid (LKB1) and compared to control overnight transfection with the pcDNA™3.3-TOPO® plasmid (Ctrl), followed by 24 h treatment under control conditions of normoxia and complete media (21%oxygen, 10% serum), serum deprivation and hypoxia (1% oxygen, 10% serum). Quantification of cell viability assessed by Crystal violet assay are shown. At least triplicates were used per experimental condition. Data is shown as mean ± SEM. **p < 0·01 is indicated (Mann-Whitney U test).