Supplemental Fig. 4.

Liver Kinase B1 (LKB1) is SUMOylated by SUMO-2 at Lys178 in mouse liver progenitor MLP-29 cells. a. Ni²⁺-NTA agarose bead pulldown in MLP-29 cells after transfection with His-SUMO-1, 2, or 3, with the pcDNA3-FLAG-LKB1 Wild type plasmid (LKB1 WT) in the presence and absence of ubiquitin conjugating enzyme 9 (UBC9). b. Ni²⁺-NTA agarose bead pulldown in Huh-7 human hepatoma cells after transfection with His-SUMO-2 and the LKB1 SUMO mutants, LKB1 K96R, LKB1 K97R, LKB1 K178R and LKB1 K235R. Normalized quantifications relative to inputs are shown below each panel.