Supplemental Fig. 6.

Cycloheximide Chase Assay. a. Stability of pcDNA3-FLAG-LKB1 wild type (WT) and b. pcDNA3-FLAG-LKB1 K178R or c. pcDNA3-FLAG-LKB1 K48R proteins as percentage of protein levels remaining after treatment with cycloheximide (CHX). Densitometric analysis and quantifications was obtained from Western blot data. LKB1 WT and mutant proteins at different time points between 0 and 6 h after CHX treatment. Vertical bars are indicative of the calculated half-life for each protein. At least triplicates were used per experimental condition. Data is shown as mean ± SEM.