. 2019 Mar 11;9:4119. doi: 10.1038/s41598-019-40815-z

Table 1.

Michaelis-Menten analysis of USP37^wt and USP37^mUIM1,2,3 for K11, K48 and K63 di-Ub substrates.

Substrate Enzyme	K11		K48		K63
Substrate Enzyme	USP37^wt	USP37^mUIM1,2,3	USP37^wt	USP37^mUIM1,2,3	USP37^wt	USP37^mUIM1,2,3
[E] (nM)	25	25	1.25	1.25	12.5	12.5
V_max (nM/min)	285 ± 22	108 ± 12	17 ± 2.1	1.1 ± 0.2	20 ± 1.2	6.0 ± 0.4
k_cat (s⁻¹)	0.19	0.072	0.23	0.015	0.027	0.008
K_M (µM)	11 ± 2.3	19 ± 1.2	0.40 ± 0.09	0.72 ± 0.16	1.7 ± 0.08	3.6 ± 0.42

V_max and K_M values are mean ± SD.