Figure 5.

Ectopic PKD2 expression is not sufficient to restore cancer cell viability after treatment with cold plasma. Cancer cells were transduced with a PKD2 expression (PKD2 o.e.) or empty vector (e.v.). After selection with antibiotic, lysates of transduced cancer cells were prepared and SDS-PAGE was conducted with PKD2 antibody. β-actin was used as loading control. (B,C) MDA-MB-231 breast and SW480 colon cancer cells (e.v. as well as PKD2 o.e.) were treated with plasma (60 s) and/or 50 nM PU-H71, and cell viability was determined at 24 h.