Temporal and phylogenetic analysis of 27 A. baumannii GC1 isolates showing the chromosomal distribution of ISAba1 and the surface polysaccharide loci, KL and OCL. Dated whole-genome phylogeny reconstructed using beast with the x-axis indicating calendar years. Nodes with black dots indicate a posterior support of <0.7 and all other nodes have support of >0.9. The tree tips are labelled with isolate names and the tip colours indicate the countries, with the key in the left corner of the figure. The KL and OC types are coloured to highlight those that differ from the inferred ancestral types KL1 and OCL1. The first two columns, on the right side of the tree, indicate KL and OCL types, followed by two columns showing the context of the oxa23 gene, with the key in the bottom left corner of the figure. The remaining columns indicate the presence/absence of ISAba1 copies in given chromosomal positions, with numbers representing the first base of the 9 bp target site duplication generated by ISAba1 insertion (numbers are normalized according to the A85 chromosome, GenBank accession no. CP021782). ISAba1 copies belonging to Tn6168 and Tn2006 are shown blue and green, respectively. Small black arrows pointing to different parts of the tree indicate the acquisition of AbaR4, Tn6168, OCL3, gyrA-3, parC-3 and phage.