Fig. 3. SOX12 promotes CRC cell tumorigenesis and metastasis in vivo.

a The indicated cells were subcutaneously injected into nude mice (n = 10 mice per group). Representative fluorescence images of luciferase signals captured from subcutaneous tumors are shown. b Growth curves of tumors in nude mice (n = 10 mice per group) injected with the indicated cells. c Tumors (n = 10) were isolated and weighed 28 days after the injection. d IHC staining for Ki67 and the percentage of Ki67-positive cells in the indicated tumors. The scale bars represent 50 µm. e Representative BLI scans of lung metastasis in the different groups at 9 weeks after implantation. f The bioluminescence intensity at the indicated time points is presented as the total photon flux. g Incidence of lung metastasis in the indicated groups of nude mice. h Overall survival time in the different groups of nude mice. i Representative images of H&E-stained lung tissues from the different groups. The scale bars represent 200 µm (upper panel) and 50 µm (lower panel). The number of lung metastatic nodules is shown. *P < 0.05 compared with the control. The data are presented as the mean ± SD