Fig. 3.

Changes in protein expression on B cells during follow-up time points. (A) Spearman's rank correlation coefficients for correlations of each antigen with time on treatment are grouped into early (months 0–3), intermediate (months 3–6) and late (months 6–36) alterations. Colour intensity represents statistical significance. Positive correlations represent relative marker expression increases, and negative correlations represent relative decreases. As examples, correlations marked with † are further explained in Fig. S6. (B) Hierarchical clustering (average linkage) of protein expression by Spearman's rank correlation coefficient. (C) Paired marker expression for the same time intervals arranged by hierarchical clustering. Only antigens showing significant changes (P < .05) in (A) were included in the heat maps. Colour intensity represents the difference between correlations of co-expression and mutually exclusive expression of antigen pairs with time on treatment. Dots denote significant differences (P < .05) of cell subsets co-expressing or mutually lacking the respective pair of markers.