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. 2018 Dec 23;40:210–223. doi: 10.1016/j.ebiom.2018.12.038

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 5 — Overexpression of YAP partly reverses the effect of S1PR3 knockdown in OS. a. Overexpression efficacy of YAPS127A (constitutively active YAP that cannot be phosphorylated by LATS kinases) in sh-S1PR3 OS cells (MNNG-HOS and U-2OS) was detected by western blotting. b and c. Overexpression of YAP partly reversed the suppressed effects of S1PR3-knockdown on the colony formation capability of MNNG-HOS and U-2OS cells, values are means ± SD, **p < .01, ***p < .001 (Student's t-test). d and e. Overexpression of YAP partly reversed the induce effect of S1PR3-knockdown on the apoptosis of OS cells (MNNG-HOS and U-2OS). Values are means ± SD, ***p < .001 (Student's t-test). f. Morphologic characteristics of xenograft tumors from MNNG-HOS/sh-Control group, MNNG-HOS/sh-S1PR3 group and MNNG-HOS/sh-S1PR3 + YAPS127A group (n = 5). Scale bars = 1 cm. g. Overexpression of YAP partly rescued the inhibitory effects of S1PR3-knockdown on the growth rate of MNNG-HOS cells in vivo. The volumes of tumors were measured every 5 days, values are means ± SD, **p < .01, ***p < .001 (Student's t-test). h. The ECAR in OS cells (MNNG-HOS and U-2OS) in different groups (sh-Control, sh-S1PR3 and sh-S1PR3 + ov-YAP) were determined. Values are means ± SD. i. The OCR in OS cells (MNNG-HOS and U-2OS) in different groups (sh-Control, sh-S1PR3 and sh-S1PR3 + ov-YAP) were determined. Values are means ± SD.