Skip to main content
. 2019 Mar 5;10:168. doi: 10.3389/fphys.2019.00168

FIGURE 4.

FIGURE 4

In vitro desialylation modulates AAV-mediated eGFP expression in NRVMs and hiPSC-CMs. Cardiomyocyte-specific eGFP expression with (+NM, 25 mU/mL) and without (−NM) neuraminidase treatment prior to viral infection in (A) NRVMs at MOI 2000 and (C) hiPSC-CMs at MOI 30,000. Cell nuclei were labeled with DAPI (blue, NRVMs only) and AAV-infected cells expressed eGFP (green). All scale bars are 50 μm and color-enhanced images are shown. Quantification of eGFP expression with and without desialylation in all three serotypes in (B) NRVMs and (D) hiPSC-CMs. eGFP expression mediated by AAV1 and 6 significantly decreased in both cell types, whereas transgene expression mediated by AAV9 significantly increased in hiPS-CMs only. Application of a higher dose of NM (500 mU/mL) in hiPSC-CMs infected with AAV9 resulted in even greater eGFP expression. Data are presented as mean ± SEM (n = 3–7 independent samples per group). ∗Significance level at p < 0.05.