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. 2019 Mar 5;10:168. doi: 10.3389/fphys.2019.00168

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Copyright © 2019 Ambrosi, Sadananda, Han and Entcheva.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The 37/67 kDa LamR is expressed in the rat heart, but not in cultured NRVMs and hiPSC-CMs. (A) Negative in vitro immunostains of NRVMs and hiPSC-CMs for LamR. Concurrent immunostaining of HeLa cells, serving as a positive in vitro control for LamR. Scale bars are 50 μm. (B) Positive immunostains of adult and neonatal rat hearts. Concurrent immunostaining of breast carcinoma tissue, serving as a positive in vivo control for LamR. (C) Negative controls of tissue (stained with no primary LamR antibody) showed no contribution to the positive stain by non-specific secondary antibody staining. Scale bars in B,C are 3 mm.