Fig. 2.

Distribution of up- and down- regulated genes according to cell source. Red: genes up-regulated; green: genes down regulated. The medians of 5 logged fold change values (base 2) between children with asymptomatic P. falciparum infection compared with healthy children (AxCo), children with uncomplicated malaria compared with children having asymptomatic infection (UxA), children with malaria associated with severe anemia compared with children having uncomplicated malaria (SxU) and children with cerebral malaria compared with children having uncomplicated malaria (CexU) were colorimetrically represented in the columns. The expression pattern of the genes is represented in the horizontal strips. The grey strips define the limits between the cell-specific groups of genes for lymphocytes (Ly), monocytes (Mo), granulocytes (Gr) and reticulocytes (Re). Arrows: gene expression patterns verified by real-time RT-PCR in samples of children with uncomplicated malaria and severe malaria associated with severe anemia. Gene expression profiles were allocated to specific cell types according to previous research done on the same platform (www.affymetrix.com/support/technical/technotes/blood_technote.pdf). Due to thrombocytopenia, a common finding in malarial disease, RNA amounts of platelets were too low to compare with those obtained from other blood cell sources (lymphocyte, monocyte, granulocyte and reticulocytes), and were thus not shown.