Table 3.
qRT-PCR primer information.
| Primer name | Gene Symbol | Reference | Primer Sequence 5′ to 3′ | Accession number |
|---|---|---|---|---|
| Reference Genes | ||||
| Ribosomal protein lateral stalk subunit P0 | RPLP0 | Passmore et al., 2009 | NM_001012682.1 | |
| Hypoxanthine phosphoribosyltransferase 1 | HPRT1 | Passmore et al., 2009 | NM_001034035.1 | |
| Tyrosine 3-monooxygenase | YWHAZ | McGillick et al., 2013 | AY970970 | |
| Growth factors and cell cycle regulators | ||||
| Insulin-like growth factor 1 | IGF1 | Zhang et al., 2010 | DQ152962 | |
| Insulin-like growth factor 1 receptor | IGF1R | Zhang et al., 2010 | AY162434 | |
| Cyclin dependent | CDKN1A | F 5′ TCAGGAGGACCACTTGGA 3′ | NM_001161880.1 | |
| kinase inhibitor 1A | R 5′ AATCTGTCATGCTGGTCTGG 3′ | |||
| Cyclin dependent kinase inhibitor 1B | CDKN1B | Lie et al., 2014 | NM_001100346.1 | |
| Neuregulin Signaling | ||||
| Neuregulin 1 | NRG1 | F 5′ GGATAGTGGAGGATGAGGAGTA 3′ | XM_012105892.2 | |
| R 5′ GTGCCTGTGTTGTCCATTTC 3′ | ||||
| Erb-b2 receptor | ERBB4 | F 5′ CCGCCTCTCTTTCTCTTGTT 3′ | XM_004004898.3 | |
| tyrosine kinase 4 | R 5′ GGTTCCTCCTCTCTTTCTCTTTC 3′ | |||
| Meis1, Hippo and FGF pathways | ||||
| Meis homeobox 1 | MEIS1 | F 5′ AGCAGTGAGCAAGGTGATG 3′ | XM_012168162.2 | |
| R 5′ CAGAAGGGTAAGGGTGTGTTAG 3′ | ||||
| Yes associated | YAP1 | F 5′ AGCAGTTGCAGATGGAGAAA 3′ | NM_001267881.2 | |
| protein 1 | R 5′ TGAGACATCCCAGGAGAAGATA 3′ | |||
| Fibroblast growth | FGF1 | F 5′ AGAACGGAAGCTCCAAACTC 3′ | XM_004008910.3 | |
| factor 1 | R 5′ AGCACGGCCAATGGTAAA 3′ | |||
| Proliferation markers | ||||
| Marker of proliferation Ki-67 | MKI67 | Orgeig et al., 2015 | XM_005197116.1 | |
| Proliferating cell nuclear antigen | PCNA | Orgeig et al., 2015 | NM_001034494.1 | |
| Inflammatory markers | ||||
| Tumor necrosis | TNFA | F 5′ ACACCATGAGCACCAAAAGC 3′ | X55152 | |
| factor | R 5′ AGGCACCAGCAACTTCTGGA 3′ | |||
| Interleukin 6 | IL6 | F 5′ TCATCCTGAGAAGCCTTGAGA 3′ | FJ409227 | |
| R 5′ TTTCTGACCAGAGGAGGGAAT 3′ | ||||
| Interleukin 1 beta | IL1β | Orgeig et al., 2015 | NM_001009465.2 | |
| Apoptosis and autophagy | ||||
| BCL2, apoptosis regulator | BCL2 | Botting et al., 2014 | HM630309.1 | |
| Beclin 1 | BECN1 | Botting et al., 2014 | XM_004012945.1 | |
| Hypertrophy markers | ||||
| Natriuretic | NPPA | F 5′ ATCACCACGAGCTTCCTCCTCTTT 3′ | NM_001160027.1 | |
| peptide A | R 5′ ATACTTGTGAGGGCACAGCCTCAT 3′ | |||
| Natriuretic | NPPB | F 5′ CCTGCTTCTCCTCTTCTTGC 3′ | NM_001160026.1 | |
| peptide B | R 5′ TAGACGGTCCAACAGCTCCT 3′ | |||
| Myosin heavy | MYH7 | F 5′ ACCAACCTGTCCAAGTTCCG 3′ | NM_174727.1 | |
| chain 7 | R 5′ GCACGGCTACTCCTCATTCA 3′ | |||
| Regulator of | RCAN1 | F 5′ CTGTCCGATGCGACCAATAA 3′ | NM_001009274.1 | |
| calcineurin 1 | R 5′ GAAACCTACGTATTGCCTGATCTA 3′ | |||
| ATPase | ATP2A2 | F 5′ GATGTCGCTCCACTTCCTAATC 3′ | XM_004017359.1 | |
| sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 | R 5′ TCCATTAGAATCACAGGCAAGG 3′ | |||
| Collagens | ||||
| Collagen type I | COL1A1 | F 5′ TTCACCTACAGCGTCACCTACGAT 3′ | FJ200442 | |
| alpha 1 chain | R 5′ ATGTCGAAGCCGAATTCCTGGTCT 3′ | |||
| Collagen type III | COL3A1 | F 5′ AACCAGAACCGTGCCAAATATGCG 3′ | NM_001076831 | |
| alpha 1 chain | R 5′ TGGGCAAACTGCACAACATTCTCC 3′ | |||
| Fatty Acid Metabolism | ||||
| Carnitine palmitoyltransferase 1 | CPT1 | Nicholas et al., 2013 | NM_001009259 | |
| Cluster of differentiation 36 | CD36 | Wang et al., 2013 | BC103112.1 | |
| Fatty acid synthase | FASN | Lock et al., 2017; Soo et al., 2017 | AF479289.1 | |
| Fatty acid binding protein | FABP | Zhang et al., 2016 | NM_001145180.1 | |
| Glucose transport and metabolism | ||||
| Solute carrier family 2 member 4 | SLC2A4 | Muhlhausler et al., 2009 | AB005283 | |
| Pyruvate dehydrogenase kinase 4 | PDK4 | Lie et al., 2014 | XM_004007738 | |