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Kinetics of expression of secreted proteins by CHO-K1 cells. Titers of (A) the secreted human erythropoietin (hEPO), (B) human granulocyte colony-stimulating factor isoform-b (hG-CSF) and, (C) secreted/cytoplasmic mouse alpha-fetoprotein (mAFP) released in serum-free culture medium of CHO-K1 cells were monitored by ELISA.
