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. 2019 Feb 6;47(5):2681–2698. doi: 10.1093/nar/gkz069

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — C3P3-G1 enzyme is a 200 kDa cytoplasmic protein. (A) Immunofluorescence of FLAGx3-NP868R-(G4S)₂-K1ERNAP C3P3-G1 enzyme is found in the cytoplasm of CHO-K1 cells, with or (B) without nuclear staining with Hoechst 33342. (C) Western blot analysis of HEK-293 and CHO-K1 cells untransfected or transfected with the FLAGx3-NP868R-(G4S)₂-K1ERNAP or FLAG-RLUC plasmid used as a positive control. The 200 kDa band corresponds to the expected molecular weight of the full-length flagged C3P3-G1 protein and the 36 kDa to the FLAG-RLUC control.