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. 2019 Feb 6;47(5):2681–2698. doi: 10.1093/nar/gkz069

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 9. — Kinetics of C3P3-G1 gene expression monitored by quantitative RT-PCR and luciferase assay. HEK-293 were either cotransfected with pCMV-NP868R-(G4S)₂-K1ERNAP and pK1E-Luciferase (i.e. Firefly Luciferase gene under control of the phage K1E promoter), or pCMVScript-Luciferase plasmids (i.e. Firefly Luciferase gene under control of the RNAP II-dependent IE1 human CMV promoter/enhancer). (A) The kinetics of pCMV-NP868R-(G4S)₂-K1ERNAP C3P3-G1 mRNA and (B) Luciferase mRNA were assayed by quantitative RT-PCR, along with (C) Firefly Luciferase expression monitored by luciferin oxidation assay.