Figure 3.

TDP-43 stabilizes scaRNA2 and suppresses the formation of mgU2-61. (A) RNAs prepared from 293T cells treated with siRNA-1, siRNA-2 or ncRNA (control) for 96 h for TDP-43 knockdown were detected by northern blotting with DNA probes shown in Figure 1C. The levels of TDP-43 were examined by western blot analysis with anti-TDP-43. Closed arrowhead indicates full-length scaRNAs. (B) The graph shows the relative band intensities of RNAs that were normalized with that of 5S rRNA detected by SYBR gold staining. Mean ± SEM, n = 3–4; *P < 0.05, **P < 0.01 by Tukey’s test versus ncRNA.