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. 2018 Dec 31;40:471–487. doi: 10.1016/j.ebiom.2018.12.057

Fig. 6.

Fig. 6

Predominant expression of G6PD- and ALDH2-clustered genes in mouse and human HCC and noncancerous cells. A. Kaplan-Meier plots for the 4-gene signature (G6PD, HIF1A, PKM, MTHFS) using RNA-seq data for 360 patients from TCGA (left), and from an independent microarray cohort with 222 patients [42,43]. B. Immunohistochemistry staining of G6PD, HIF1A, PFKP, CAT, and MTHFD1 through antibodies HPA000834, HPA001275, HPA018257, HPA055838, and HPA050052, respectively [25,27]. Protein expression is displayed in blue, with counterstaining in blue. C. Gene expression profile in murine samples revealed substantially higher expression of G6PD cluster genes in HCC than noncancer samples and higher expression in CB1R+/+ (hypoxia-associated) compared with CB1R−/− samples, compared with the expression of genes in the ALDH2 cluster. mRNA expression profile measured in six pooled wild-type HCC tumor and noncancerous samples using RNA-seq in CB1R+/+ and CB1R−/− six murine samples. For all boxplots, whiskers extended up to 1.5 times the interquartile range. D. The distribution of differences for genes in the two clusters in HCC and noncancerous (NC) murine samples. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)