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. 2019 Feb 19;20(4):895. doi: 10.3390/ijms20040895

Figure 1.

Figure 1

The cyclic GMP-AMP synthase (cGAS) pathway is activated in bone marrow-derived dendritic cells (BMDCs) during Mycobacterium bovis infection. (A) BMDCs were treated with siRNA, siCon, and M. bovis, and cGAS protein was analyzed by Western blotting at 24 h after treatment. (B) The related proteins in the cGAS pathway in BMDCs were assayed by Western blotting. The protein levels of cGAS, p-STING, STING, TBK1, and p-TBK1 were analyzed in BMDCs transfected with siCon or sicGAS and then infected for 24 or 48 h with M. bovis (MOI 5). (C) The co-localization of IRF3 within the nucleus was detected by immunofluorescence microscopy (400 ×). (D) The culture supernatants were harvested after 24 h and assessed by ELISA. All data are expressed as mean ± SD, (* p < 0.05; ** p < 0.01; n.s.: no statistical significance). IFN: interferon.