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. 2019 Feb 25;11(2):483. doi: 10.3390/nu11020483

Figure 5.

Figure 5

Ergosterol alleviated extracellular matrix (ECM) accumulation. (A) Representative renal sections with immunohistochemical staining of fibronectin and collagen I. (B) Expression of fibronectin, collagen I, MMP-2, and MMP-9 mRNA levels in renal parenchyma (data are presented as the means ± SD, n = 3). (C) Expression of MMP-2 and MMP-9 protein levels in renal parenchyma through Western blotting (data are presented as the means ± SD, n = 3). (D) Expression levels of fibronectin, collagen I, MMP-2 and MMP-9 in RMCs cultured under different conditions through Western blotting (data are presented as the means ± SD, triplicate). NC: normal control; DN: diabetic nephropathy model induced by STZ; ENA: positive control, 1.5 mg/kg/day enalaprilat administration; ERG10, ERG20, ERG40: 10, 20, 40 mg/kg/day ergosterol administration, respectively. NG: normal glucose, 5.6 mM glucose; Mannitol: 5.6 mM glucose + 24.4 mM mannitol; HG: high glucose, 30 mM glucose; ERG10 and ERG 20: 10 and 20 μM ergosterol, respectively. ** p < 0.01, *** p < 0.001 compared with NG or NC; # p < 0.05, ## p < 0.01, ### p < 0.001 compared with HG or DN.