Table 1.
CRISPR-Cas9-edited genes in O. sativa L.
| Application Prospective | Target Gene | Cas9 Version | Cas9 Promoter | sgRNA Promoter | Transformation Method | Reference |
|---|---|---|---|---|---|---|
| Quality Improvement | Waxy | N/A | 35S | OsU6 | Agrobacterium-mediated transformation | [64] |
| SBE1, SBEIIb | Codon-optimized Cas9 | ZmUbi | OsU3 | [65] | ||
| ISA1 | Rice codon-optimized | 35S | OsU6 | [66] | ||
| OsPDS, OsBADH2, Oso2g23823, OsMPK2 | Rice codon-optimized | 2 × 35S | OsU3 | [67] | ||
| OsCYP97A4, OsDSM2, OsCCD4a, OsCCD4b, and OsCCD7 | Rice codon-optimized | 35S | OsU3 | [68] | ||
| OsNramp5 | Rice codon-optimized | CaMV35S | OsU6a | [69] | ||
| Yield Improvement | Gn1a, DEP1, GS3, IPA1 | Codon-optimized Cas9 | OsUbi | OsU6a | Agrobacterium-mediated transformation | [70] |
| GLW2 | Plant codon-optimized | 2 × 35S | OsU6 | [70] | ||
| GS9 | Rice codon-optimized | CaMV 35S | OsU3 | [71] | ||
| GW2, GW5 and TGW6 | Codon-optimized Cas9 | OsUbi | OsU3, OsU6 and TaU3 | [72] | ||
| TMS5 | Codon-optimized Cas9 | OsU3/U6 | OsU3/U6 | [73] | ||
| Disease resistance | OsERF922 | Codon-optimized Cas9 | CaMV 35S | OsU6 | Agrobacterium-mediated transformation | [74] |
| Bsrk1 | Rice codon-optimized | 35S | OsU6 | [75] | ||
| ALS | Rice codon-optimized | 2 × P35S | OsU6 | [76] | ||
| OsAnn3 | Codon-optimized Cas9 | CaMV35S | OsU6 | [77] |