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. 2019 Feb 21;11(2):448. doi: 10.3390/nu11020448

Figure 1.

Figure 1

Inhibition of gas production assay. The lower layer corresponds to the RCM agar inoculated with Clostridium butyricum ATCC 860 and the upper layer is MRS medium with 0.7% agar and 100 mM phosphate buffer inoculated with Bifidobacterium and Lactobacillus strains; cultures were incubated at 37 °C for 24 h under anaerobic conditions. (a) Gas production by C. butyricum in buffered MRS medium, indicating the absence of inhibitory activity of Lactobacillus and Bifidobacterium strains. (b) Inhibitory activity of five Lactobacillus and two Bifidobacterium strains on gas production by Clostridium butyricum.