Phenotypes of near-isogenic lines and chromosome segment substitution lines. (a) Plant morphology at tillering stage; bar = 18 cm. (b) Plant morphology at heading stage; bar = 18 cm. (c) The top three leaf shape of NIL-qLL9CJ16 and NIL-qLL9C84. From left to right are the first leaf, the second leaf, and the third leaf, respectively; bar = 5 cm. Comparison between the NIL-qLL9CJ16 and NIL-qLL9C84 for the flag leaf length (d), the flag leaf width (e), and the flag leaf area (f). Comparison between Wuyunjing (WYJ) 7 and chromosome segment substitution line (CSSL)-qLL9KASA for the flag leaf length (g), the flag leaf width (h), and the flag leaf area (i). Data are mean ± SD (n = 15). Asterisks represent significant difference determined by Student’s t-test at p-value < 0.01 (**).