Table 3.
Primers for QTL fine mapping, quantitative real-time polymerase chain reaction (qRT-PCR), vector construction, and gene editing.
| Primer | Forward (5′-3′) | Reverse (5′-3′) | Experiment |
|---|---|---|---|
| RM3700 | AAATGCCCCATGCACAAC | TTGTCAGATTGTCACCAGGG | Fine mapping |
| C9-1594 | CCTGTACACTGTAGGCCTGT | GGTGTCAAAGTACATAGGCCC | |
| C9-1635 | GGTGGAAAGGAAGGAGAGCT | CTAGCCCTGCCTCGTTGTAA | |
| C9-1638 | GTGTGTGTGTGTGTGTGTGT | TCATAGTACATGCCCTCCGT | |
| C9-1640 | ATAAGTCCATATTGCCCACCTC | AAGCTTCTGGATCGTTAACAGG | |
| C9-1642 | GTACCCTCCTCCGATGACAC | TTGTGGAGGACGAGAAGGTG | |
| C9-1715 | GGTGGCGAGAAGAATTTGCA | TTTCGCCTCTCACTGACCTT | |
| B9-11 | TCTTACGAATAGGCCCTTGG | AGAGCCCACAACACTTGTGC | |
| Actin | ATCCATCTTGGCATCTCTCAGC | CACAATGGATGGGCCAGACT | qRT-PCR |
| LOC_Os09g26960 | CTGAGCCTCGCCAATCTG | CGAAGATCTCCTCCATGCTC | |
| LOC_Os09g26970 | CAAACATCTGGGCTTGGTCT | TCTAAGCAACCTGCCCAATC | |
| LOC_Os09g26980 | ATTGATGTGAAAGGGCAAGACT | CACCTTAAGCCCAAGGTTGTAG | |
| LOC_Os09g26999 | GTAGCTGCAAGCCAAGCTG | TTGAAGCAGCTGGAGCAAC | |
| POs26999 | GGCCAGTGCCAAGCTTAAGGGAAGTTGGCCGCCTGCC | AGGGTCTTGCAGATCTCTCCACACGCAGCACGCCAAC | Vector construction |
| Os09g26999-g++/g-- | GGCAGGTGGTGATGGAGGCGCCG | AAACCGGCGCCTCCATCACCACC | |
| Os09g26999-JC | CGGCGATTTATACCCACCAC | CGCTCACCTTGAGGAACGT | Detection of target mutations |
| Hyg-F1 | GCTGTTATGCGGCCATTGTC | GACGTCTGTCGAGAAGTTTC | |
| Cas9-F2/pC1300-R2 | ACCAGACACGAGACGACTAA | ATCGGTGCGGGCCTCTTC | |
| T3 | ATCGGTGCGGGCCTCTTC |