Table 2.
Excerpt of pathways altered in PBMC of piglets fed diets with low and high amounts of P and Ca. Pathways were selected for affiliation with immune response, biosynthesis, and signaling. A full list of pathways is displayed in Table S4.
| Pathway Name | z-Score | Responsive Transcripts |
|---|---|---|
| Biosynthesis | ||
| Superpathway of Inositol Phosphate Compounds * | - | ACP1, DUSP2, IPMK, PIK3C2A, PIP5K1B, PPP1R12A, PTEN, PTPN12, SET, SOCS3, TNS3 |
| EIF2 Signaling * | 1.13 | ATF3, EIF3K, PIK3C2A, RPL12, RPL7, RPL24, RPL34, RPS8, RPS19, RPS26, RPSA, SOS2 |
| CDP-diacylglycerol Biosynthesis I † | - | CDS1, GPAT3, GPAT4, LPCAT2, MBOAT7 |
| Triacylglycerol Biosynthesis † | - | DGAT2, GPAT3, GPAT4, LPCAT2, MBOAT7, PLPP3 |
| Interferon Signaling * | 2.00 | IFIT3, IFNGR1, JAK2, OAS1 |
| Immune Response | ||
| IL-10 Signaling ‡ | - | ARG2, CCR1, HMOX1, IL1A, IL1R2, IL1RAP, IL4R, IL18, IL18RAP, SOCS3 |
| IL-6 Signaling ‡ | 2.89 | CSNK2A1, IL1A, IL1R2, IL1RAP, IL6R, IL18, IL18RAP, JAK2, PIK3C2A, SOCS3, SOS2, TNFRSF1A |
| Acute Phase Response Signaling ‡ | 2.11 | C4BPA, F8, HMOX1, IL1A, IL1RAP, IL6R, IL18, JAK2, RIPK1, SOCS3, SOD2, SOS2, TNFRSF1A |
| NF-κB Signaling † | 3.05 | BMPR2, CSNK2A1, IL1A, IL1R2, IL18, IRAK4, PIK3C2A, RIPK1, TGFA, TGFBR1, TLR4, TNFRSF1A, TNFSF13B |
| Th1 and Th2 Activation Pathway † | - | BMPR2, CCR1, CD247, CD274, IFNGR1, IL4R, IL6R, IL18, JAK2, NOTCH2, PIK3C2A, SOCS3, TGFBR1 |
| T Helper Cell Differentiation † | - | BCL6, IFNGR1, IL4R, IL6R, IL18, TGFBR1, TNFRSF1A |
| Th1 Pathway * | 1.41 | CD247, CD274, IFNGR1, IL6R, IL18, JAK2, NOTCH2, PIK3C2A, SOCS3 |
| Fcγ Receptor mediated Phagocytosis in Macrophages and Monocytes † | 2.12 | ARPC2, FGR, GAB2, HCK, HMOX1, NCF1, PTEN, PTK2B |
| Granulocyte Adhesion and Diapedesis † | - | CCL14, CSF3R, CXCR2, IL1A, IL1R2, IL1RAP, IL18, IL18RAP, ITGAM, SELL, TNFRSF1A |
| Role of Pattern Recognition Receptors in Recognition of Bacteria and Viruses * | 2.00 | C3AR1, DDX58, IL1A, IL18, NOD1, OAS1, PIK3C2A, TLR4 |
| LXR/RXR Activation ‡ | 2.53 | ABCA1, ARG2, FDFT1, IL1A, IL1R2, IL1RAP, IL18, IL18RAP, LY96, MYLIP, TLR4, TNFRSF1A |
| LPS/IL-1 Mediated Inhibition of RXR Function † | 1.89 | ABCA1, ACSL1, ACSL4, ALDH1L2, GSTM3, IL1A, IL1R2, IL1RAP, IL18, IL18RAP, LY96, RARA, TLR4, TNFRSF1A |
| PPAR Signaling * | 2.65 | IL1A, IL1R2, IL1RAP, IL18, IL18RAP, SOS2, TNFRSF1A |
| Signaling | ||
| RhoA Signaling * | 0.38 | ABL2, ARHGAP6, ARPC2, LIMK2, LPAR6, PIP5K1B, PPP1R12A, PTK2B |
| p38 MAPK Signaling † | 2.83 | IL1A, IL1R2, IL1RAP, IL18, IL18RAP, IRAK4, RPS6KA3, TGFBR1, TNFRSF1A |
| TREM1 Signaling * | 2.45 | CASP5, IL18, JAK2, NOD1, TLR4, TREM1 |
| iNOS Signaling * | 2.24 | IFNGR1, IRAK4, JAK2, LY96, TLR4 |
The significance of association between the data set and pathway: * p < 0.05; † p < 0.01; ‡ p < 0.001. Positive and negative z-scores suggest activation or inhibition, respectively. Missing z-scores have not been calculated due to lack of database information. Gene symbols in bold font: H > L; gene symbols in normal font: H < L. EIF—Eukaryotic initiation factor, CDP—Cytidine diphosphate, IL—Interleukin, NF-κB—Nuclear factor κB, Th—T helper cell, LXR—Liver X receptor, RXR—Retinoid X receptor, LPS—Lipopolysaccharide, PPAR—Peroxisome proliferator-activated receptors, RhoA—Ras homolog gene family, member A, MAPK—Mitogen-activated protein kinase, TREM1—Triggering receptor expressed on myeloid cells 1, iNOS—Inducible nitric oxide synthase.