Figure 1.

Three types of methods and designs for microfluidic single-cell droplet generation. Adapted by permission from Reference [28], copyright Royal Society of Chemistry 2015. (a) T-junction microchannel based droplet generation for encapsulating single cells for cultivation and genomic analysis. Adapted by permission from reference [49], under the Creative Commons Attribution License 2017. (b) Flow-focusing for encapsulating a single cell and a barcoding hydrogel bead in a droplet for single-cell barcoding and sequencing. Adapted by permission from Reference [27], copyright Nature Publishing Group 2017. (c) Co-flow for preparing multiple component double emulsions. Adapted by permission from Reference [50], copyright Royal Society of Chemistry 2012.