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. 2019 Feb 1;10(2):104. doi: 10.3390/mi10020104

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Genetic analysis of single-cells by using microfluidics. (a) An integrated microfluidic chip for chromosome enumeration using FISH. Adapted by permission from Reference [109], copyright Royal Society of Chemistry 2008. (b) High-throughput microfluidic single-cell RT-qPCR for gene expression analysis. Adapted by permission from Reference [110], copyright National Academy Sciences 2011. (c) Capturing single-cells along with a set of uniquely barcoded primers in microfluidic chip generated tiny droplets enables single-cell transcriptomics of many cells in a heterogeneous population. Adapted by permission from Reference [30], copyright Elsevier Inc. 2015.