Figure 2.

Physosmotic culture medium (PM) increases TGF-β2 secretion and TGF-β bioactivity in HACs. In HACs cultured for seven days (serum-free cultures on the left and 10% FCS cultures on the right) TGF-β2 protein levels were determined by specific TGF-β2 ELISA in PM (black bars) or OCM (white bars) (A). TGF-β bioactivity was determined by TGF-β bioassay in 72 h serum starved conditioned medium from PM and OCM HAC cultures (B). Corresponding ALK5/ALK1 gene expression ratio was determined in samples from Figure 1 (C). Data are from 3 donors measured in duplicate and are presented as mean ± standard deviation relative to control OCM conditions.