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. 2019 Feb 13;20(4):795. doi: 10.3390/ijms20040795

Figure 5.

Figure 5

Blocking of BMP signalling decreases COL2A1 expression of chondrocytes irrespective of osmolarity. Bioassays in SW1353 cells show a largely TGF-β2 knockdown-independent, but osmolarity-dependent induction of BMP-responsiveness. Data are the normalized means ± standard deviation (n = 3) relative to control OCM condition (A). Physosmolar conditions further significantly elevated COL2A1 expression as compared to vehicle controls at OCM (B). Dorsomorphin (10 μM)-mediated inhibition of BMP signaling significantly suppressed COL2A1 expression in both osmolarities (B), but COL2A1 expression remained relatively higher in PM as compared to OCM irrespective of BMP signaling. Combining TGF-β2 knockdown and dorsomorphin showed the same pattern as dorsomorphin alone (C). Data from five donors were measured in duplicates and are presented as normalized means ± standard deviation relative to control OCM condition. Data is normalized means ± standard relative to control OCM condition (replicate measurements, three donors).