Figure 3.

BCAS2 colocalizes with ERα on estrogen regulated promoters upon incubation with E2 and promotes increased estrogen-regulated gene expression. (A,B) Chromatin immunoprecipitation was performed in MCF7 cells after different times of exposure to E2 (10 nM) treatment (30–180 min). Statistical analysis was carried out comparing ER or BCAS2 with IgG control using by one-way ANOVA, * p < 0.001. We carried out qRT-PCR of precipitated DNA showing endogenous ERα and BCAS2 localization at promoter regions of pS2 and WISP2 genes. Additionally, Histone 3 methylation (H3k4me3) is shown as a marker for open chromatin. (C–F) MCF7 cells transfected with pcDNA-BCAS2 or empty vector (EV) and incubated in the presence (+) or absence (−) of 10nM E2 for 24h. RTqPCR was used to determine mRNA expression of pS2, C3, insulin-like growth factor binding protein 2 (IGFBP2) and C-MYC and the results were normalized against human GAPDH mRNA amplification. * p < 0.05 compared to the EV group at 24 h in the absence (−) or presence of E2, as indicated in the figure.