Figure 7.

ILF-RE inhibits CCl₄-induced ER stress and lipogenesis. Primary hepatocytes were treated with 20 mM CCl₄ in the absence or presence of 50 and 100 μg/mL ILF-RE for 6 h. (A) Immunoblotting was performed using anti-CHOP, anti-p-PERK, anti-PERK, anti-p-eIF2α, anti-eIF2α, or anti-β-actin antibodies. Quantification of immunoblot data was performed (lower panel). (B) Immunoblotting was performed using anti-SREBP-1, anti-FAS, and anti-β-actin antibodies. Quantification of immunoblot data was performed (lower panel). Data are expressed relative to the controls (n = 3; ^# p < 0.05 vs. Con; * p < 0.05 vs. CCl₄). Con, control; CCl₄, carbon tetrachloride; ILF-RE, the combination R. verniciflua with E. ulmoides; ER, endoplasmic reticulum.