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. 2019 Feb 7;41(4):2137–2147. doi: 10.3892/or.2019.7004

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Copyright: © Shen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — BHLHE41 knockdown inhibits the cell proliferation and migration of ccRCC cells. (A) The inhibition effect of BHLHE41 knockdown on cell proliferation was examined by WST-1 in A498 cells. (B) The inhibition effect of BHLHE41 knockdown on cell proliferation was examined by WST-1 in CAKI-1 cells. (C) BrdU assay in A498 cells. (D) BrdU assay in CAKI-1 cells. (E) Colony formation assay in A498 cells. (F) Colony formation assay in CAKI-1 cells. (G) BHLHE41 knockdown also decreased the migration cell number in ccRCC cells. Scale bar, 100 µm. All the experiments were repeated 3 times, and the central horizontal line represented the mean. *P<0.05. ccRCC, clear cell renal cell carcinoma; BHLHE41, basic helix-loop-helix family member e41; NC, negative control; shRNA, short hairpin RNA; O.D., optical density; WST-1, water-soluble tetrazolium salt.