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Classification. The first generation (G1) uses a tRNACUA carrying a standard natural amino acid. In contrast, an unnatural aminoacyl-tRNA synthetase/tRNACUA pair suppresses UAGs in the second generation (G2). The third generation (G3) is an upgraded G2 that achieves the best performance for switching. Figure 4 illustrates G3 in detail. Std.aa, a standard amino acid. Uaa, an unnatural amino acid. UaaRS, unnatural aminoacyl-tRNA synthetase.
