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Two components of the leakage translation. The leakage translation attributed to the switch (LTaS) is mainly caused by misacylation of tRNACUA with a natural amino acid, which is a poor substrate of UaaRS. The leakage translation attributed to the basal read-through (LTaBR) is derived from read-through caused by near-cognate tRNAs. The LTaBR is independent of the performance of the Uaa translational switch.
