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. 2019 Feb 18;20(4):887. doi: 10.3390/ijms20040887

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© 2019 by the author.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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G3. Various relevant factors for switching are optimized in G3, such as (1) inducible UaaRS/tRNA_CUA, (2) optimization of UaaRS/tRNA_CUA expression level, (3) multiplexed UAG insertion in a target gene, (4) location of the UAG insertion, (5) Uaa-residue converting to a standard amino acid residue, (6) suppression of peptide release factor, (7) Uaa-permissive elongation factor, (8) host selection, and (9) improved UaaRS/tRNA_CUA. Asterisk indicates a mutant optimized for switching use. UAAon, a transcriptional ON-switch controlled by Uaa.