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. 2019 Feb 23;24(4):813. doi: 10.3390/molecules24040813

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of PRFR on HepG2 cells apoptosis. The cells were incubated with or without PRFR (0–40 µg/mL) for 48 h. Cell apoptosis was determined using Guava Nexin analysis (a) Annexin V-PE positive cells indicated early apoptosis, while double positive cells indicated late apoptosis. The percentages in early and late apoptosis were summed up; all assays were performed in triplicate in three independent experiments and the mean ± standard deviations are shown as the histogram (b). **** p < 0.0001 versus the control.

Effect of PRFR on HepG2 cells apoptosis. The cells were incubated with or without PRFR (0–40 µg/mL) for 48 h. Cell apoptosis was determined using Guava Nexin analysis (a) Annexin V-PE positive cells indicated early apoptosis, while double positive cells indicated late apoptosis. The percentages in early and late apoptosis were summed up; all assays were performed in triplicate in three independent experiments and the mean ± standard deviations are shown as the histogram (b). **** p < 0.0001 versus the control.