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. 2019 Jan 30;41(4):2117–2125. doi: 10.3892/or.2019.6989

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — TSPAN1 modulates MMP2 expression via PLCγ. SW1990 and BxPC3 cells were transfected with siRNAs targeted to TSPAN1 (siRNA group) and pLNCX-TSPAN1-cDNA recombinant plasmid (TSPAN1 group). (A) RT-qPCR and western blotting were performed to assess the expression of (a and b) MMP2 and (c) MMP9 at the mRNA and protein level following TSPAN1 silencing in SW1990 and BxPC3 cells. Scrambled siRNA-transfected cells were used as controls (Ctrl). (B) The mRNA and protein expression of (a and b) MMP2 and (c) MMP9 was assessed following TSPAN1 overexpression in the indicated cells. Plasmid pLNCX transfected into SW1990 and BxPC3 cells was used as the control (Ctrl). (C) NIH3T3 cells were transfected with PLCγ siRNA. RT-qPCR and western blotting was performed to detect the expression of PLCγ. Following PLCγ siRNA transfection, the expression of MMP2 was detected in TSPAN1 overexpressed (D) SW1990 and (E) BxPC3 cells. *P<0.05 and **P<0.01 vs. Ctrl. TSPAN1, Tetraspanin 1; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; siRNA, small interfering RNA; Ctrl, control; MMP, matrix metalloproteinase; PLCγ, phospholipase Cγ.

Figure 4. — TSPAN1 modulates MMP2 expression via PLCγ. SW1990 and BxPC3 cells were transfected with siRNAs targeted to TSPAN1 (siRNA group) and pLNCX-TSPAN1-cDNA recombinant plasmid (TSPAN1 group). (A) RT-qPCR and western blotting were performed to assess the expression of (a and b) MMP2 and (c) MMP9 at the mRNA and protein level following TSPAN1 silencing in SW1990 and BxPC3 cells. Scrambled siRNA-transfected cells were used as controls (Ctrl). (B) The mRNA and protein expression of (a and b) MMP2 and (c) MMP9 was assessed following TSPAN1 overexpression in the indicated cells. Plasmid pLNCX transfected into SW1990 and BxPC3 cells was used as the control (Ctrl). (C) NIH3T3 cells were transfected with PLCγ siRNA. RT-qPCR and western blotting was performed to detect the expression of PLCγ. Following PLCγ siRNA transfection, the expression of MMP2 was detected in TSPAN1 overexpressed (D) SW1990 and (E) BxPC3 cells. *P<0.05 and **P<0.01 vs. Ctrl. TSPAN1, Tetraspanin 1; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; siRNA, small interfering RNA; Ctrl, control; MMP, matrix metalloproteinase; PLCγ, phospholipase Cγ.