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. 2019 Feb 21;20(4):946. doi: 10.3390/ijms20040946

Figure 3.

Figure 3

The histological structures of the colons in Lop-induced constipated rats after SPA administration: (A) The hematoxylin and eosin (H&E)-stained sections of the colon from the CNTR, SPA-, Lop + Vehicle- or Lop + SPA-treated group were observed at 100× (left column) and 200× (left column) using a light microscope.(B) The histopathological parameters were determined using the Leica Application Suite (Leica Microsystems). Five to six rats per group were used in the histological analysis, and each parameter was measured in duplicate in two different slides. The data are reported as the mean ± SD. * indicates p < 0.05 compared to the CNTR group. # indicates p < 0.05 compared to the Lop + Vehicle-treated group.