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. 2019 Feb 21;20(4):946. doi: 10.3390/ijms20040946

Figure 7.

Figure 7

The detection of mucin secretion and membrane water channel expression in the colon after SPA treatment: (A) Mucin secreted from the crypt layer cells was stained with Alcian Blue at pH 2.5, and the images were observed at 100× magnification. Five to six rats per group were used in the preparation of the tissue slide and mucin secretion observed in duplicate in three stained slides. (B) The levels of MUC2 and AQP8 transcripts in the total mRNA of the colons were measured by RT-PCR using specific primers. After the intensity of each band was determined using an imaging densitometer, the relative levels of MUC2 and AQP8 mRNA were calculated based on the intensity of actin as an endogenous control. Four to five rats per group were used in RNA purification, and each transcript levels were assayed in duplicate in each sample. The data are reported as the mean ± SD. * indicates p < 0.05 compared to the CNTR group. # indicates p < 0.05 compared to the Lop + Vehicle-treated group.