Table 3.
Plasma parameters, relative weight of liver, cholesterol and triglycerides content, neutrophils infiltration, and collagen content in LDLr-KO mice fed experimental diets for 16 weeks.
| PUFA | PALM | PALM INTER | STEAR | STEAR INTER | |
|---|---|---|---|---|---|
| ALT (mg/dL) | 37.7 ± 12.4 | 61.3 ± 7.6 | 51.6 ± 12.2 | 48.4 ± 14.4 | 58.0 ± 14.1 |
| Glucose (mg/dL) | 232 ± 13.0 b | 336 ± 33.9 a | 328 ± 27.6 a | 211 ± 20.1 b | 288± 15.7 b |
| Insulin (mg/dL) | 0.3 ± 0.0 b | 0.8 ± 0.1 a | 0.7 ± 0.1 a | 0.3 ± 0.1 b | 0.2 ± 0.0 b |
| Liver weight (g/100g body weight) | 4.4 ± 0.6 | 4.7 ± 1.1 | 4.5 ± 0.6 | 4.5 ± 0.4 | 4.4 ± 0.8 |
| Cholesterol (g/100g liver) | 1.7 ± 0.3 | 2.1 ± 0.6 | 1.7± 0.4 | 1.7 ± 0.4 | 1.9 ± 0.5 |
| Triglycerides (g/100g liver) | 15.1 ± 7.7 | 16.7 ± 11.2 | 15.6 ± 5.4 | 16.5 ± 8.4 | 13.5 ± 5.4 |
| Neutrophils infiltration (cell/mm2) | 1.8 ± 0.8 b | 2.3 ± 0.9 b | 3.0 ± 1.9 b | 2.0 ± 0.8 b | 6.1 ± 3.9 a |
| Collagen content CLV * (%) | 24.3 ± 0.8 b | 14.7 ± 7.3 b | 34.8 ± 20.3 a | 15.2 ± 9.9 b | 31.6 ± 13 a |
| Collagen content triad (%) | 25.9 ± 9.9 b | 31.8 ± 14.2 b | 51.2 ± 9.7 a | 32.0 ± 14.9 b | 36.6 ± 18.6 b |
Liver samples were dissected from LDLr-KO mice fed a high-fat diet enriched with polyunsaturated fatty acids (PUFA), palmitic acid (PALM), palmitic interesterified fat (PALM INTER), stearic acid (STEAR), and stearic interesterified fat (STEAR INTER) for 16 weeks. Data were checked for normality prior to statistical analysis. One-way ANOVA was performed, followed by the post hoc Newman–Keuls Multiple Comparison Test. ALT and glucose Kruskal–Wallis were performed, followed by post hoc Dunn. Statistically significant differences are represented by different letters (p < 0.05) with a > b values. Data are shown as mean ± SD (n = 14−19). Neutrophils were counted in 20 fields of the hepatic parenchyma under 40x magnification (n = 8–10). * CLV: Centrilobular vein.