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Scutellarein (SCU) induces apoptosis in Hep3B cells. To quantify the extent of SCU-induced apoptosis, the cells were untreated or treated with SCU at indicated concentrations (100-, 200-, and 300-μM) for 24 h. Allophycocyanin (APC)/Annexin V and propidium iodide (PI) double-staining was performed which was analyzed by flow cytometry. Values are given as the mean ± standard error of the mean (SEM). ** p < 0.01 vs. Hep3B cell control, *** p < 0.001 vs. Hep3B cell control.
