Figure 6.

Impact of extraction methods on enzymes involved in cannabinoid biosynthesis identified by bottom-up proteomics. (A) Cannabinoid pathway. Enzymes in red were identified in this work. (B) Two-dimensional hierarchical clustering of enzymes involved in cannabinoid synthesis. Columns represent extraction method per tissue types (AB, apical bud; T, trichomes. See Column legend as well) and rows represent the peptides identified from enzymes of interest. Peptides from the same enzymes bear the same shade of grey (see Row legend as well). CBDA, Cannabidiolic acid; CBDAS, CBDA synthase; CBGA, Cannabigerolic acid; GOT, geranyl-pyrophosphate-olivetolic acid geranyltransferase; OAC, Olivetolic acid cyclase; OLA, Olivetolic acid; OLS, 3,5,7-trioxododecanoyl-CoA synthase; THCA, delta9-tetrahydrocannabinolic acid; THCAS, THCA synthase.