Table 1.
Protein extraction efficiency of the six methods tested on cannabis apical buds and trichomes assessed by top-down proteomics.
| Tissue | Extraction Number | Extraction Method | Extraction Code | Protein Concentration (mg/mL) | Number of Proteins | ||
|---|---|---|---|---|---|---|---|
| Average (SD 1) | Average (SD 1) | CV 2 | Percent | ||||
| apical bud | extraction 1 | Urea | AB1 | 6.58 (± 0.89) | 254 (± 12) | 4.80 | 44.51 |
| apical bud | extraction 2 | Gnd-HCl | AB2 | 3.50 (± 0.99) | 335 (± 15) | 4.47 | 58.58 |
| apical bud | extraction 3 | TCA-A/urea | AB3 | 0.63 (± 0.15) | 247 (± 21) | 8.69 | 43.23 |
| apical bud | extraction 4 | TCA-A/Gnd-HCl | AB4 | 1.50 (± 0.28) | 314 (± 16) | 5.13 | 54.90 |
| apical bud | extraction 5 | TCA-E/urea | AB5 | 0.60 (± 0.11) | 201 (± 5) | 2.64 | 35.11 |
| apical bud | extraction 6 | TCA-E/Gnd-HCl | AB6 | 0.76 (± 0.48) | 264 (± 18) | 6.84 | 46.18 |
| trychome | extraction 1 | Urea | T1 | 3.67 (± 0.39) | 170 (± 5) | 2.97 | 29.83 |
| trychome | extraction 2 | Gnd-HCl | T2 | 2.28 (± 1.17) | 249 (± 45) | 18.12 | 43.61 |
| TOTAL | 571 | ||||||
1. SD, standard deviation; 2 CV, coefficient of variation.