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. 2019 Feb 20;24(4):756. doi: 10.3390/molecules24040756

Figure 3.

Figure 3

(A) Effect of PDGF-BB capture aptamer concentration on the response of LFA. PDGF-BB concentration: 25 nM; assay time: 10 min; sample solutions were prepared with PBST + 1% BSA; dispensing cycles of gold nanoparticle (AuNP)-aptamer conjugate on the conjugate pad: 4. (B) Effect of dispensing cycles of AuNP-aptamer conjugate on the response of LFA. PDGF-BB concentration: 25 nM; Assay time: 10 min; sample solution was prepared with PBST + 1% BSA; concentration of capture aptamer for the preparation of PDGF-BB test line: 4 OD/mL. (C) Effect of running buffer components on the response of LFA. PDGF-BB concentration: 25 nM; assay time: 10 min; concentration of capture aptamer for the preparation of PDGF-BB test line: 4 OD/mL; dispensing cycles of AuNP-aptamer conjugate: 4.