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(A) Response histogram of the LFA test lines in the presence of thrombin and PDGF-BB at different concentrations. Assay time: 10 min; concentration of capture aptamers for the preparation of test lines: 4 OD/mL; cycles of dispensing AuNP-aptamer conjugates: 4; sample solutions were prepared with PBST + 1% BSA. (B) Calibration curve of the LFA for the detection of PDGF-BB. (C) Calibration curve of the LFA with for the detection of thrombin.
