Figure 3.

Schemes regarding the effects of UVB irradiation on TIMP3 expression depending on treatment. (A) Schemes for irradiation. After synchronization (Sync) of NHEKs by being incubated in a serum-rich medium (keratinocyte basal medium, KBM, supplemented with 50% horse serum) for 2 h, 20 mJ/cm² of UVB was used for treatment immediately (Scheme I) or 12 h (Scheme II) post-synchronization; (B,C) NHEKs were irradiated following each scheme and harvested every 4 h. TIMP3 expression was analyzed in each condition by qRT-PCR, showing differences between the control and UVB-treated groups following Scheme II; CON indicates control.